Bioluminescence

Bioluminescence has proven an elemental tool in biological research. Scientists have harnessed the power of a naturally-occurring bioluminescent reaction into reporter assays where a luciferase enzyme produces light upon the addition of a light-emitting substrate. Here, we have a superior collection of luciferin substrates for luciferase imaging in animal models and cell-based assays. In addition, we have developed the necessary resources to further facilitate your gene network and signaling pathway research including relaible luciferase assay protocols, guides and comprehensive handbooks for luciferin substrate use in vitro and in vivo.

Select Luciferin Products

GoldBio offers a wide range of luciferin substrates including Firefly D-Luciferin salts (Proven and Published®), ideal for luciferase imaging in mice, cell culture and ATP assays. We also offer Coelenterazine, which can be used in BRET (Bioluminescence Resonance Energy Transfer), ELlSA, high-throughput screening assays and the chemiluminescent detection of superoxide anions and peroxynitrite in cells and tissues.

GoldBio also offers the highly sensitive single and dual Illumination™ Assay Kits that allow measurement of firefly and renilla activity in vitro with high sensitivity and linearity and come in both flash & glow-type formats for your different detection and emission duration needs. In addition, GoldBio’s Illumination™ Assay Kits are also available in lyophilized form for your convenience. Here, you will also find different luciferin assay lysis buffer options as well as various supporting reagents.

SUPPORTING REAGENTS

– Penicillin G Sodium Salt, USP Grade
– G418 Sulfate
– Sterile Filtered DMSO
– Carbenicillin (Disodium) 100 mg/mL Solution
– Carbenicillin (Disodium) EZ Pak™ for 100 mg/mL Solution
– Kanamycin Monosulfate EZ Pak™ for 50 mg/mL Solution
– Hygromycin B EZ Pak™ for 50 mg/mL Solution
– Kanamycin Monosulfate 50 mg/mL Solution
– Ampicillin (Sodium) EZ Pak™ for 100 mg/mL Solution
– PBS (Phosphate Buffered Saline) Tablets
– Ampicillin (Sodium) 100 mg/mL Solution
– Adenosine-5′-Triphosphate (ATP), Disodium trihydrate, Ultra Pure
– Puromycin Dihydrochloride
– Penicillin G Potassium Salt, USP Grade
– Hygromycin B
– Kanamycin Monosulfate, USP Grade
– Sodium Pyruvate
– NADH (β-Nicotinamide Adenine Dinucleotide Reduced Sodium Salt)
– HEPES, Free Acid
– Carbenicillin (Disodium), USP Grade
– HEPES, Sodium Salt
– Ampicillin (Sodium), USP Grade

Discover Luciferin Resources

Our complete collection of luciferin resources contains detailed information to help you succesfully carry out your luciferase assay experiments. Our luciferase assay kit comparison guide and fact sheet offers information on our products to help you decide the best luciferase substrate for your system. In addition, we offer detailed protocols for each of our Illumination™ assay kits including our Firefly & Renilla Enhanced Assay. We also provide you with comprehensive in vitro and in vivo D Luciferin handbooks containg specific instructions for luciferase activity measurement and imaging in cell culture and animal models.