1 Kb RNA Ladder (lyophilized)

The Norgen 1kb RNA ladder is a set of RNA transcripts derived from recombinant DNA templates. This ladder is suitable for precise sizing of a wide range of RNA molecules using native or denaturing agarose gels, and can be visualized under UV.

Contents:

• 2 vials of lyophilized RNA ladder (25 loads each).
• 1x loading buffer: 33.3% formamide, 11.7% formaldehyde, 0.012%
• bromophenol blue, 0.05mM EDTA, 0.012% ethidium bromide, and 0.7x MOPS solution.
• 2x loading buffer.

Specifications:

• No need for staining and destaining of denaturing gels since Norgen’s loading buffer contains ethidium bromide
• Highly stable
• nine bands from 200 bases to 4000 bases
• Convenient lyophilized format provides better product stability

Instructions for Use:

To reconstitute the lyophilized RNA ladder, add 250 µL of 1x loading buffer to each 25 loads vial and vortex gently.
Heat at 80°C for 10 minutes. Incubate on ice for 1 min. Load 10 µL on a 1-2% gel.