PNPG (Gus)

PNPG (Gus) is a chromogenic β-glucuronidase substrate, yielding a yellow solution upon cleavage. It is used to detect E. coli contamination
in food and drinking water due to their β-glucuronidase activity.

Product Specifications
PNPG (Gus);
4-Nitrophenyl-β-D-glucuronic acid;
p-Nitrophenyl-β-D-glucuronide;

PNP-β-D-GlcA

MOLECULAR BIOLOGY GRADE

Formula: C₁₂H₁₃NO₉

MW: 315.240 g/mol

Storage/Handling: Store desiccated at -20°C. Protect from light.

References

1. Kilian, M., & Bülo, P. (1976). Rapid diagnosis of Enterobacteriaceae: I. Detection of bacterial glycosidases. Acta Pathologica Microbiologica Scandinavica Section B Microbiology, 84(5), 245-251.
2. Le Minor, L. (1979). Tetrathionate reductase, beta-glucuronidase, and ONPG-test in the genus Salmonella (author’s transl). Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie, 243(2-3), 321.
3. Kilian, M., & Bülow, P. (1979). Rapid Identification of Enterobacteriaceae: II. Use of a β‐glucuronidase Detecting Agar Medium (PGUA Agar) for the Identification of E. coli in
   Primary Cultures of Urine Samples.
   Acta Pathologica Microbiologica Scandinavica Section B Microbiology, 87(1‐6), 271-276.
4. Manafi, M., Kneifel, W., & Bascomb, S.(1991). Fluorogenic and chromogenic substrates used in bacterial diagnostics. Microbiological reviews, 55(3), 335-348.
5. Nimmo-Smith, R. H. (1961). p-Nitrophenyl-beta-glucuronide as substrate for beta-glucuronidase. Biochimica et biophysica acta, 50, 166.