Description
TCEP (Tris (2-Carboxyethyl) phosphine Hydrochloride) is an odorless reducing agent suitable for protein applications and in the preparation for electrophoresis. TCEP is water soluble, is stable in a variety of solutions and is particularly useful in the labeling of cysteine residues with maleimides, preventing the cysteines from forming disulfide bonds.
TCEP 50 g and 100 g ships as multiple 25 g units to qualify for a small quantity exception to hazardous
fees.
Due to hazardous goods restrictions, this product cannot ship by ground shipping to Canada or Mexico.
Benefits
- PROTEASE FREE Oxidizing agent
- TCEP-HCl is stable in air.
- TCEP-HCl is odorless so reactions can be carried out on the bench instead of the fume hood.
- TCEP-HCl is efficient and can be used to reduce disulfide bonds at low concentrations.
- TCEP-HCl is selective for disulfides and does not react with other protein side chains.
- TCEP-HCl may be used as a substitute for DTT at a final concentration of 50mM.
Storage/Handling
Store desiccated at -20°C.
Working Concentration
TCEP is commonly stored in a 0.5M stock solution for up to 3 months at -20°C.
Working concentrations are typically around 5-50mM and should be remade daily.
