Description
Detection principle
Acid phosphatase decomposes disodium phenyl phosphate under acidic conditions to produce free phenol and phosphoric acid. Phenol acts with 4-aminoantipyrine in alkaline solution, and oxidizes to a derivative of red quinone by potassium ferricyanide. The activity of the ACP can be calculated by measuring the OD value at 520 nm.
Performance characteristics
Synonyms | ACP |
Sample type | Serum,plasma,hydrothorax,urine,cells,cell culture supernatant,animal tissue |
Sensitivity | 1.40 U/100 mL |
Detection range | 1.40-40 U/100 mL |
Detection method | Colorimetric method |
Assay type | Enzyme Activity |
Assay time | 45 min |
Precision | Average inter-assay CV: 9.4%Average intra-assay CV: 2.1% |
Other instruments required | Micropipettor, Centrifuge, Incubator, Vortex mixer |
Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Storage | 2-8℃ |
Valid period | 3 months |