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Acid Phosphatase (ACP) Activity Assay Kit

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Description

Detection principle

Disodium p-nitrobenzene phosphate (p-NPP), a widely used phosphatase chromogenic substrate, can form p- nitrophenol under the action of acid phosphatase. Under alkaline conditions, p-nitrophenol is yellow and has a maximum absorption peak at 405 nm. The darker of the yellow product is, the higher of the ACP activity is. Therefore, the activity of ACP can be calculated by measuring the OD value at 405 nm.

Performance characteristics

Synonyms ACP
Sample type Serum, plasma, tissue
Sensitivity 0.2 U/L
Detection range 0.2–50 U/L
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 30 min
Precision Average inter-assay CV: 4.5%Average intra-assay CV: 2.8%
Other instruments required Micropipettor, 37℃ Incubator
Other reagents required PBS (0.01 M, pH 7.4)
Storage -20℃
Valid period 3 months

Manual MSDS

Additional information

CatalogID

E-BC-K010-M-500, E-BC-K010-M-96