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Acid Phosphatase (ACP) Activity Assay Kit

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Description

Detection principle

Acid phosphatase decomposes disodium phenyl phosphate under acidic conditions to produce free phenol and phosphoric acid. Phenol acts with 4-aminoantipyrine in alkaline solution, and oxidizes to a derivative of red quinone by potassium ferricyanide. The activity of the ACP can be calculated by measuring the OD value at 520 nm.

Performance characteristics

Synonyms ACP
Sample type Serum,plasma,urine,tissue,cells
Sensitivity 0.27 U/100 mL
Detection range 0.27-40 U/100 mL
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 70 min
Precision Average inter-assay CV: 7.1%Average intra-assay CV: 2.8%
Other instruments required Micropipettor, Incubator, Vortex mixer, Centrifuge
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 3 months

Manual MSDS

Additional information

CatalogID

E-BC-K094-S-100, E-BC-K094-S-500