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Alanine Aminotransferase (ALT/GPT) Activity Assay Kit (Reitman-Frankel Method)

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Description

Detection principle

ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37℃. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.

Performance characteristics

Synonyms ALT,GPT,Glutamic-pyruvic transaminase
Sample type Serum,plasma,cells,animal tissue
Sensitivity 1.26 IU/L
Detection range 1.26-72.3 IU/L
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 100 min
Precision Average inter-assay CV: 8.199999999999999%Average intra-assay CV: 4.3%
Other instruments required Micropipettor, Vortex mixer, Incubator
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 6 months

Manual MSDS

Additional information

CatalogID

E-BC-K235-S-100, E-BC-K235-S-50