Description
Detection principle
ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37℃. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.
Performance characteristics
| Synonyms | ALT,GPT,Glutamic-pyruvic transaminase |
| Sample type | Serum,plasma,cells,animal tissue |
| Sensitivity | 1.26 IU/L |
| Detection range | 1.26-72.3 IU/L |
| Detection method | Colorimetric method |
| Assay type | Enzyme Activity |
| Assay time | 100 min |
| Precision | Average inter-assay CV: 8.199999999999999%Average intra-assay CV: 4.3% |
| Other instruments required | Micropipettor, Vortex mixer, Incubator |
| Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage | 2-8℃ |
| Valid period | 6 months |
