Description
Detection principle
ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37℃. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.
Performance characteristics
Synonyms | ALT,GPT,Glutamic-pyruvic transaminase |
Sample type | Serum,plasma,animal tissue,cells,cell culture supernatant |
Sensitivity | 0.75 IU/L |
Detection range | 0.75-72.3 IU/L |
Detection method | Colorimetric method |
Assay type | Enzyme Activity |
Assay time | 80 min |
Precision | Average inter-assay CV: 9%Average intra-assay CV: 5.3% |
Other instruments required | Micropipettor, Vortex mixer, Incubator, Multichannel pipette |
Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Storage | 2-8℃ |
Valid period | 6 months |