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Alanine Aminotransferase (ALT/GPT) Activity Fluorometric Assay Kit

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Description

Detection principle

ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid. Under the action of pyruvate oxidase, pyruvic acid generates H2O2, which reacts with the non-fluorescent substance to form fluorescent substance under the action of peroxidase. The activity of ALT can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.

Performance characteristics

Synonyms ALT
Sample type Serum, plasma, animal tissue
Sensitivity 0.01 U/L
Detection range 0.01-0.83 U/L
Detection method Fluorescence method
Assay type Enzyme Activity
Assay time 75 min
Precision Average inter-assay CV: 9.800000000000001%Average intra-assay CV: 2.3%
Other instruments required Pipettor, Vortex mixer, Centrifuge
Storage -20℃
Valid period 6 months

Manual MSDS

Additional information

CatalogID

E-BC-F038-48, E-BC-F038-96