Description
Detection principle
Bromocresol green (BCG) is widely used as protein staining agent. BCG can combine the albumin in pH 4.0~4.2 to form an albumin-BCG complex. And the color changed from yellow to green. The depth of color is proportional to the concentration of albumin. The content of albumin in serum can be calculated indirectly by measuring the OD value at 630 nm.
Performance characteristics
Synonyms | ALB |
Sample type | Serum,plasma |
Sensitivity | 0.08 g/L |
Detection range | 0.08-15 g/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 20 min |
Precision | Average inter-assay CV: 4.6%Average intra-assay CV: 1.5% |
Other instruments required | Micropipettor, Vortex mixer |
Other reagents required | Normal saline (0.9% NaCl) |
Storage | Reagent 1: 2-8℃, Reagent 2: -20℃ |
Valid period | 6 months |