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Albumin (ALB) Colorimetric Assay Kit (Bromocresol Green Method)

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Description

Detection principle

Bromocresol green (BCG) is widely used as protein staining agent. BCG can combine the albumin in pH 4.0~4.2 to form an albumin-BCG complex. And the color changed from yellow to green. The depth of color is proportional to the concentration of albumin. The content of albumin in serum can be calculated indirectly by measuring the OD value at 630 nm.

Performance characteristics

Synonyms ALB
Sample type Serum,plasma
Sensitivity 0.08 g/L
Detection range 0.08-15 g/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 20 min
Precision Average inter-assay CV: 4.6%Average intra-assay CV: 1.5%
Other instruments required Micropipettor, Vortex mixer
Other reagents required Normal saline (0.9% NaCl)
Storage Reagent 1: 2-8℃, Reagent 2: -20℃
Valid period 6 months

Manual MSDS

Additional information

CatalogID

E-BC-K057-M-48, E-BC-K057-M-96