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Albumin (ALB) Colorimetric Assay Kit (Bromocresol Green Method)

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Description

Detection principle

Bromocresol green (BCG) can combine with the albumin in pH 4.0~4.2 to form an albumin-BCG complex, which is yellowish-green. The depth of yellowish-green is proportional to the concentration of albumin. The serum albumin concentration can be calculated by measuring the OD value at 628 nm.

Performance characteristics

Synonyms ALB
Sample type Serum,plasma
Sensitivity 0.50 g/L
Detection range 0.50-70 g/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 50 min
Precision Average inter-assay CV: 4.2%Average intra-assay CV: 2.1%
Other instruments required Micropipettor, Vortex mixer
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage Reagent 1: 2-8℃, Reagent 2: -20℃
Valid period 6 months

Manual MSDS

Additional information

CatalogID

E-BC-K057-S-100, E-BC-K057-S-50