Description
Detection principle
Bromocresol green (BCG) can combine with the albumin in pH 4.0~4.2 to form an albumin-BCG complex, which is yellowish-green. The depth of yellowish-green is proportional to the concentration of albumin. The serum albumin concentration can be calculated by measuring the OD value at 628 nm.
Performance characteristics
Synonyms | ALB |
Sample type | Serum,plasma |
Sensitivity | 0.50 g/L |
Detection range | 0.50-70 g/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 50 min |
Precision | Average inter-assay CV: 4.2%Average intra-assay CV: 2.1% |
Other instruments required | Micropipettor, Vortex mixer |
Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Storage | Reagent 1: 2-8℃, Reagent 2: -20℃ |
Valid period | 6 months |