Description
Detection principle
ADH catalyzes the oxidative dehydrogenation of ethanol. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of ADH can be calculated by measuring the change of absorbance value at 450 nm.
Performance characteristics
| Synonyms | ADH |
| Sample type | serum, plasma, animal tissue |
| Sensitivity | 0.29 U/L |
| Detection range | 0.29-248 U/L |
| Detection method | Colorimetric method |
| Assay type | Enzyme Activity |
| Assay time | 30 min |
| Precision | Average inter-assay CV: 4.5%Average intra-assay CV: 4.2% |
| Other instruments required | Incubator, centrifuge, Microplate reader |
| Other reagents required | Normal saline (0.9% NaCl) |
| Storage | -20℃ |
| Valid period | 3 months |
