Description
Detection principle
Alkaline phosphatase decompose benzene disodium phosphate to produce free phenol and phosphoric acid. Phenol react with 4-aminopyrline in alkaline solution and oxidizes with potassium ferricyanide to form red quinone derivative. The enzyme activity can be calculated indirectly by measuring the OD value.
Performance characteristics
Synonyms | ALP |
Sample type | Serum,plasma,urine,tissue,cells |
Sensitivity | 0.2 King unit/100 mL |
Detection range | 0.2-55.6 King unit/1 |
Detection method | Colorimetric method |
Assay type | Enzyme Activity |
Assay time | 60 min |
Precision | Average inter-assay CV: 5.6%Average intra-assay CV: 2.1% |
Other instruments required | Micropipettor, Incubator, Vortex mixer, Centrifuge |
Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Storage | 2-8℃ |
Valid period | 6 months |