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Alkaline Phosphatase (ALP) Activity Assay Kit (PNPP method)

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Description

Detection principle

Alkaline phosphatase (ALP) is a group of cytomembrane-related enzymes with hydrolysis and transfer activity, acting on a variety of phosphate substrates. ALP is a homologous dimerase and each catalytic site contains three metal ions. There are four isozymes in humans: tissue nonspecific ALP, intestinal ALP, placental ALP and genital cell ALP.

Performance characteristics

Synonyms ALP
Sample type Serum, plasma, animal tissue, cells
Sensitivity 0.27 U/L
Detection range 0.27-50.8 U/L
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 30 min
Precision Average inter-assay CV: 4.6%Average intra-assay CV: 1.2%
Other instruments required Micropipettor, Water bath, Incubator
Other reagents required Normal saline (0.9% NaCl)
Storage -20℃
Valid period 6 months

Manual MSDS

Additional information

CatalogID

E-BC-K009-M-500, E-BC-K009-M-96