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Aspartate Aminotransferase (AST/GOT) Activity Assay Kit (Reitman-Frankel Method)

248.00

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Description

Detection principle

AST enables alpha-ketoglutaric acid and aspartic acid to displace amino to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine (DNPH) to form 2,4-dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity.

Performance characteristics

Synonyms AST,GOT
Sample type Serum, plasma, animal tissue
Sensitivity 0.38 IU/L
Detection range 0.38-72.30 IU/L
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 100 min
Precision Average inter-assay CV: 4.9%Average intra-assay CV: 4.2%
Other instruments required Test tube, Micropipettor, 37℃ Water bath, Vortex mixer
Other reagents required Normal saline (0.9% NaCl)
Storage 2-8℃
Valid period 6 months

Manual MSDS

Additional information

CatalogID

E-BC-K236-S-100