Description
Detection principle
AST enables alpha-ketoglutaric acid and aspartic acid to displace amino to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine (DNPH) to form 2,4-dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity.
Performance characteristics
Synonyms | AST,GOT |
Sample type | Serum, plasma, animal tissue |
Sensitivity | 0.38 IU/L |
Detection range | 0.38-72.30 IU/L |
Detection method | Colorimetric method |
Assay type | Enzyme Activity |
Assay time | 100 min |
Precision | Average inter-assay CV: 4.9%Average intra-assay CV: 4.2% |
Other instruments required | Test tube, Micropipettor, 37℃ Water bath, Vortex mixer |
Other reagents required | Normal saline (0.9% NaCl) |
Storage | 2-8℃ |
Valid period | 6 months |