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Aspartate Aminotransferase (AST/GOT) Activity Assay Kit (Reitman-Frankel Method)

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Description

Detection principle

AST/GOT enables alpha-ketoglutaric acid and aspartic acid to displace amino and keto groups to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine(DNPH) to form 2,4, dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity.

Performance characteristics

Synonyms AST,GOT,Glutamic-oxalacetic transaminase
Sample type Serum,plasma,animal tissue,cells,cell culture supernatant
Sensitivity 1.1 IU/L
Detection range 1.1-72.3 IU/L
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 80 min
Precision Average inter-assay CV: 6.8%Average intra-assay CV: 5.3%
Other instruments required Micropipettor, Vortex mixer, Incubator, Multichannel pipette
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 6 months

Manual MSDS

Additional information

CatalogID

E-BC-K236-M-48, E-BC-K236-M-96