Description
Detection principle
AST/GOT enables alpha-ketoglutaric acid and aspartic acid to displace amino and keto groups to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine(DNPH) to form 2,4, dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity.
Performance characteristics
Synonyms | AST,GOT,Glutamic-oxalacetic transaminase |
Sample type | Serum,plasma,animal tissue,cells,cell culture supernatant |
Sensitivity | 1.1 IU/L |
Detection range | 1.1-72.3 IU/L |
Detection method | Colorimetric method |
Assay type | Enzyme Activity |
Assay time | 80 min |
Precision | Average inter-assay CV: 6.8%Average intra-assay CV: 5.3% |
Other instruments required | Micropipettor, Vortex mixer, Incubator, Multichannel pipette |
Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Storage | 2-8℃ |
Valid period | 6 months |