Description
Detection principle
Cu2+ can be reduced to Cu+ by protein in alkaline condition. Cu+ can combine with BCA reagent and form purple complex, which has a maximum absorption peak at 562 nm. The absorbance value is proportional to the protein concentration. Therefore, the protein concentration can be calculated according to the OD value.
Performance characteristics
Synonyms | TP |
Sample type | Serum,plasma,cell culture supernatant,tissue,cells |
Sensitivity | 0.0165 mg/mL |
Detection range | 0.0165-1 mg/mL |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 40 min |
Precision | Average inter-assay CV: 4.5%Average intra-assay CV: 2.2% |
Other instruments required | Vortex mixer, Micropipettor, Incubator |
Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Storage | RT |
Valid period | 12 months |