FREE SHIPPING IN GERMANY ON ORDERS OVER € 500 & € 1000 ACROSS EUROPE!

BCA Protein Colorimetric Assay Kit

From: 138.00

SKU: N/A Category:

Description

Detection principle

Cu2+ can be reduced to Cu+ by protein in alkaline condition. Cu+ can combine with BCA reagent and form purple complex, which has a maximum absorption peak at 562 nm. The absorbance value is proportional to the protein concentration. Therefore, the protein concentration can be calculated according to the OD value.

Performance characteristics

Synonyms TP
Sample type Serum,plasma,cell culture supernatant,tissue,cells
Sensitivity 0.0165 mg/mL
Detection range 0.0165-1 mg/mL
Detection method Colorimetric method
Assay type Quantitative
Assay time 40 min
Precision Average inter-assay CV: 4.5%Average intra-assay CV: 2.2%
Other instruments required Vortex mixer, Micropipettor, Incubator
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage RT
Valid period 12 months

Manual MSDS

Additional information

CatalogID

E-BC-K318-M-500, E-BC-K318-M-96