Description
Detection principle
Any compound that contains two -CONH2 in the molecule can react with alkaline copper solution to form a purple complex, which is known as the biuret reaction. Many peptide bonds (-CONH-) in protein molecules can perform this reaction, and the color degree of all kinds of proteins are essentially the same.
Performance characteristics
| Synonyms | TP |
| Sample type | Serum,plasma,tissue |
| Sensitivity | 0.58 g/L |
| Detection range | 0.58-100 g/L |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 20 min |
| Precision | Average inter-assay CV: 6.5%Average intra-assay CV: 4% |
| Other instruments required | Micropipettor, Incubator, Vortex mixer, Centrifuge |
| Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage | Reagent 3: -20℃, others: 2-8℃ |
| Valid period | 6 months |
