Bluo-Gal is used as an alternative to X-Gal, and produces a darker blue color. Bluo-Gal is hydrolyzed by β-galactosidase to produce a blue precipitate. This precipitate is insoluble in alcohol and xylenes, thus making it suitable for immunoblotting and immunocytochemical assays. Bacterial colonies containing active β-galactosidase produce blue colonies when grown on plates containing Bluo-Gal.
Bluo-Gal is commonly used in conjunction with IPTG (GoldBIo #I2481) for blue-white screening.
MOLECULAR BIOLOGY GRADE
MW: 374.18 g/mol
Purity: >99% by HPLC
Soluble in dimethylformamide (DMF)
Storage/Handling: Store at -15°C, and protect from light.
PubChem Chemical ID: 44161406
|Grade||MOLECULAR BIOLOGY GRADE|
|Storage/Handling||Store at -20°C. Protect from light.|