Bluo-Gal

Bluo-Gal is used as an alternative to X-Gal, and produces a darker blue color. Bluo-Gal is hydrolyzed by β-galactosidase to produce a blue precipitate. This precipitate is insoluble in alcohol and xylenes, thus making it suitable for immunoblotting and immunocytochemical assays. Bacterial colonies containing active β-galactosidase produce blue colonies when grown on plates containing Bluo-Gal.

Bluo-Gal is commonly used in conjunction with IPTG (GoldBIo #I2481) for blue-white screening.

Product Specifications:
Bluo-Gal
5-Bromo-3-indolyl-β-D-galactopyranoside
Blue-Gal, Lapis™-β-D-Gal

MOLECULAR BIOLOGY GRADE

Formula: C₁₄H₁₆BrNO₆

MW: 374.18 g/mol

Purity: >99% by HPLC

Soluble in dimethylformamide (DMF)

Storage/Handling: Store at -15°C, and protect from light.

PubChem Chemical ID: 44161406