Description
Detection principle
Calcium ion in the sample bind to Methyl Thymol Blue (MTB) in alkaline solution and form blue complex. The blue complex has a specific absorption peak at 715nm and calcium content can be calculated by measuring the OD value at 610 nm.
Performance characteristics
Synonyms | Ca |
Sample type | Serum,plasma,urine,cell culture supernatant,tissue,cells |
Sensitivity | 0.07 mmol/L |
Detection range | 0.07-1.2 mmol/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 20 min |
Precision | Average inter-assay CV: 8.5%Average intra-assay CV: 4.7% |
Other instruments required | Micropipettor, Centrifuge, Incubator, Vortex mixer |
Storage | 2-8℃ |
Valid period | 6 months |