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Catalase (CAT) Activity Assay Kit

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Description

Detection principle

The reaction that catalase (CAT) decomposes H2O2 can be quickly stopped by ammonium molybdate. The residual H2O2 reacts with ammonium molybdate to generate a yellowish complex. CAT activity can be calculated by production of the yellowish complex at 405 nm.

Performance characteristics

Synonyms CAT
Sample type Serum,plasma,cells,tissue
Sensitivity 0.27 U/mL
Detection range 0.27-155.4 U/mL
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 70 min
Precision Average inter-assay CV: 5.1%Average intra-assay CV: 3.1%
Other instruments required Micropipettor, Vortex mixer, Incubator or Water-bath
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 6 months

Manual MSDS

Additional information

CatalogID

E-BC-K031-S-100, E-BC-K031-S-50