Description
Detection principle
The reaction that catalase (CAT) decomposes H2O2 can be quickly stopped by ammonium molybdate. The residual H2O2 reacts with ammonium molybdate to generate a yellowish complex. CAT activity can be calculated by production of the yellowish complex at 405 nm.
Performance characteristics
Synonyms | CAT |
Sample type | Serum,plasma,cells,cell culture supernatant,tissue |
Sensitivity | 1.12 U/mL |
Detection range | 1.12 -150 U/mL |
Detection method | Colorimetric method |
Assay type | Enzyme Activity |
Assay time | 40 min |
Precision | Average inter-assay CV: 7.7%Average intra-assay CV: 3.9% |
Other instruments required | Micropipettor, Vortex Mixer, Incubator |
Other reagents required | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) |
Storage | 2-8℃ |
Valid period | 6 months |