Description
Detection principle
The reaction that catalase (CAT) decomposes H2O2 can be quickly stopped by ammonium molybdate. The residual H2O2 reacts with ammonium molybdate to generate a yellowish complex. CAT activity can be calculated by production of the yellowish complex at 405 nm.
Performance characteristics
Synonyms | CAT |
Sample type | Serum,plasma,cells,tissue |
Sensitivity | 0.27 U/mL |
Detection range | 0.27-155.4 U/mL |
Detection method | Colorimetric method |
Assay type | Enzyme Activity |
Assay time | 70 min |
Precision | Average inter-assay CV: 5.1%Average intra-assay CV: 3.1% |
Other instruments required | Micropipettor, Vortex mixer, Incubator or Water-bath |
Other reagents required | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) |
Storage | 2-8℃ |
Valid period | 6 months |