Description
Detection principle
Under the action of acidic solution and reductant, ferric ions can be separated from transferrin in serum, and reduced into ferrous ions (Fe2+). The latter then bind to bipyridine and form pink complexes. The concentration of iron can be calculated by measuring the OD value at 520 nm indirectly.
Performance characteristics
| Sample type | Serum, plasma, animal tissue |
| Sensitivity | 0.08 mg/L |
| Detection range | 0.08-20 mg/L |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 45 min |
| Precision | Average inter-assay CV: 3.2%Average intra-assay CV: 3% |
| Other instruments required | Test tubes, Vortex Mixer, Centrifuge, Water bath, Test tubes, Vortex Mixer, Centrifuge, Water bath |
| Storage | 2-8℃ |
| Valid period | 6 months |
