Description
Detection principle
Glutathione peroxidase (GSH-Px) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG). The activity of glutathione peroxidase can be expressed by the rate of enzymatic reaction. The activity of glutathione can be calculated by measuring the consumption of reduced glutathione. Hydrogen peroxide (H2O2) and reduced glutathione can react without catalysis of GSH-Px, so the portion of GSH reduction by non-enzymatic reaction should be subtracted. GSH can react with dinitrobenzoic acid to produce 5-thio-dinitrobenzoic acid anion, which showed a stable yellow color. Measure the absorbance at 412nm, and calculate the amount of GSH.
Performance characteristics
| Synonyms | GSH-PX |
| Sample type | Serum,plasma,cells,cell culture supernatant,tissue |
| Sensitivity | 17.17 U |
| Detection range | 17.17-518.32 U |
| Detection method | Colorimetric method |
| Assay type | Enzyme Activity |
| Assay time | 40 min |
| Precision | Average inter-assay CV: 6.2%Average intra-assay CV: 2.4% |
| Other instruments required | Micropipettor, Incubator, Vortex mixer, Centrifuge |
| Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage | 2-8℃ |
| Valid period | 6 months |
