High Resolution Agarose (For Nucleotides < 1kb)

Molecular Biology Grade
For high resolution of DNA / PCR fragments < 1000 bp.
Agarose is a natural product that forms an inert matrix used in electrophoresis, chromatography and other molecular biology and biochemistry techniques. Likewise, it is neutral and easily derivatizable, so it is easy to bind to its structure proteins like enzymes, antigens or antibodies. Toxicity absence makes working with agarose very convenient.

Applications

• Molecular Screening
• High resolution gels for <1000 bp fragments
• Blotting

Technical Specifications
High Resolution Agarose (For Nucleotides < 1kb)

EEO (Electroendomosis): ≤0.12
Sulfate: ≤0.11%
Gel Strength (1%): ≥1500 g/cm2
Gelling Temperature: 35.5±1.5°C
Melting Temperature: 80±1.5°C
DNase/RNase Activity: None Detected
DNA Resolution ≥1000 bp: Finely Resolved
Gel Background: Very Low
DNA Binding: Very Low

Features

• High resolution of short PCR products and DNA fragments
• Improved clarity of gel, enhancing visibility
• Better handling than competitive products because of a stronger gel structure and higher gel strength. The chances of gels breaking or cracking when handled are greatly minimized, even with lower concentrations of agarose.
• High gel strength makes it suitable for blotting

Choosing an agarose:

Below is a table to help you choose which agarose gel type is suited for your experimental needs.

Agarose volume chart

The volume needed for your agarose gel depends on the size of your gel casting tray. Mini gels carried out on small trays use 40 mL agarose gel. The table below shows agarose gel tray dimensions and a corresponding volume range.