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Illumination™ Renilla Luciferase Enhanced Assay Kit

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Description

GoldBio’s Illumination™ Renilla Luciferase Enhanced Assay Kit is specifically designed for the measurement of Renilla luciferase in transfected cell reporter gene assays. This kit is enhanced with a water soluble coelenterazine that is easier to use and store than other conventional coelenterazine products.

Renilla luciferase is a commonly used bioluminescent reporter gene that is used to study gene regulation and function both in vivo as well as in vitro. Renilla luciferase is a monomeric 36 kDa protein isolated from the sea pansy, Renilla reniformis, which catalyzes coelenterazine with oxygen to produce a blue light (480 nm). There is no need for any post-translational modifications and the protein may be used as a gene reporter immediately after translation. Our Enhanced Assay Kit includes a special buffer which is designed to yield reliable, linear measurements of Renilla luciferase activity with maximum sensitivity and minimal autoluminescence. This kit is a flash-type luminescent assay, and requires measurement immediately after adding the substrate to the sample.

One Assay can be performed in a single tube or an individual well on a 96 well plate. The kits include enough Passive Lysis Buffer to perform the stated number of assays in plate sizes ranging from 96-well to 24-well. If needed, additional 5X Passive Lysis Buffer (GoldBio Catalog # L-745) can be purchased separately.

Kit components

  • 5X Passive Lysis Buffer
  • Renilla Luciferase Assay Buffer
  • GoldBio Enhanced Coelenterazine

Storage/Handling: Store the kit at -80°C (5X Passive Lysis Buffer and Enhanced Coelenterazine may be stored separately at -20°C).

Product may be shipped on blue ice without reducing performance. Please place at the recommended storage conditions upon receipt of the product.

Catalog ID I-925
Storage/Handling Store the kit at -80°C.
P Protocol
O Other
C Certificate of Analysis
S Safety Data Sheet

Additional information

CatalogID

I-925-1000, I-925-150, I-925-50