Description
GoldBio’s Illumination™ Renilla Luciferase Enhanced Assay Kit is specifically designed for the measurement of Renilla luciferase in transfected cell reporter gene assays. This kit is enhanced with a water soluble coelenterazine that is easier to use and store than other conventional coelenterazine products.
Renilla luciferase is a commonly used bioluminescent reporter gene that is used to study gene regulation and function both
in vivo as well as in vitro. Renilla luciferase is a monomeric 36 kDa protein isolated from the sea pansy, Renilla reniformis, which catalyzes coelenterazine with oxygen to produce a blue light (480 nm). There is no need for any post-translational modifications and the protein may be used as a gene reporter immediately after translation. Our Enhanced Assay Kit includes a special buffer which is designed to yield reliable, linear measurements of Renilla luciferase activity with maximum sensitivity and minimal autoluminescence. This kit is a flash-type luminescent assay, and requires measurement immediately after adding the substrate to the sample.
One Assay can be performed in a single tube or an individual well on a 96 well plate. The kits include enough Passive Lysis Buffer to perform the stated number of assays in plate sizes ranging from 96-well to 24-well. If needed, additional
5X Passive Lysis Buffer (GoldBio Catalog # L-745) can be purchased separately.
Kit components
- 5X Passive Lysis Buffer
- Renilla Luciferase Assay Buffer
- GoldBio Enhanced Coelenterazine
Storage/Handling: Store the kit at -80°C (5X Passive Lysis Buffer and Enhanced Coelenterazine may be stored separately at -20°C).
Product may be shipped on blue ice without reducing performance. Please place at the recommended storage conditions upon receipt of the product.
