Description
Detection principle
Lactate dehydrogenase catalyzes the reaction of lactic acid with NAD+ to produce pyruvic acid and NADH. NADH, under the action of PMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, LDH activity can be quantified by measure the OD value at 450 nm.
Performance characteristics
| Synonyms | LDH |
| Sample type | cells |
| Detection method | Colorimetric method |
| Assay type | Enzyme Activity |
| Assay time | 30 min |
| Other instruments required | Microplate centrifuge, Micropipettor, Water bath |
| Storage | -20℃ |
| Valid period | 6 months |
