Description
Detection principle
LAP can catalyze the substrate L-leucine-4-nitroaniline to produce p-nitroaniline, which has the maximum absorption peak at the wavelength of 405 nm. The enzyme activity of LAP can be calculated by measuring the increasing OD value of the system.
Performance characteristics
| Synonyms | LAP |
| Sample type | Serum, plasma, animal tissue |
| Sensitivity | 5.2 U/L |
| Detection range | 5.2-201.8 U/L |
| Detection method | Colorimetric method |
| Assay type | Enzyme Activity |
| Assay time | 25 min |
| Precision | Average inter-assay CV: 6.4%Average intra-assay CV: 4.1% |
| Other instruments required | Micropipettor, Water bath, Centrifuge, Incubator, Vortex mixer |
| Other reagents required | Acetone |
| Storage | 2-8℃ |
| Valid period | 6 months |
