Description
Detection principle
Myeloperoxidase reduces hydrogen peroxide to a complex. The complex react with o-dianisidine (as hydrogen donor) to produce a yellow product which has a maximum absorption peak at 460 nm. The activity of MPO can be calculated indirectly by measuring the OD value at 460nm.
Performance characteristics
Synonyms | MPO |
Sample type | Serum,plasma,milk,animal tissue,cells |
Sensitivity | 4.9 U/L |
Detection range | 4.9-196.7 U/L |
Detection method | Colorimetric method |
Assay type | Enzyme Activity |
Assay time | 90 min |
Precision | Average inter-assay CV: 9.800000000000001%Average intra-assay CV: 4.5% |
Other instruments required | Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge |
Storage | 2-8℃ |
Valid period | 6 months |