Description
Detection principle
Under the condition of weak acidity, non-esterified free fatty acids (NEFA) react with nantokite to form copper soap, which has a specific absorption peak at 715nm. The content of NEFA can be calculated by measuring the OD value at 715 nm.
Performance characteristics
| Synonyms | NEFA |
| Sample type | Serum,animal tissue,cells |
| Sensitivity | 0.05 mmol/L |
| Detection range | 0.05-2.0 mmol/L |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 60 min |
| Precision | Average inter-assay CV: 6.1%Average intra-assay CV: 2.2% |
| Other instruments required | Vortex mixer, Micropipettor |
| Storage | 2-8℃ |
| Valid period | 6 months |
