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Total Superoxide Dismutase (T-SOD) Activity Assay Kit (Hydroxylamine Method)

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Description

Detection principle

The superoxide anion free radical (O2-) can be produced by xanthine and xanthine oxidase reaction system, O2- oxidize hydroxylamine to form nitrite, it turn to purple under the reaction of developer. When the measured samples containing SOD, the SOD can specifically inhibit superoxide anion free radical (O2-). The inhibitory effect of SOD can reduce the formation of nitrite, the absorbance value of sample tube is lower than control tube. Calculate the SOD of sample according to the computational formula.

Performance characteristics

Synonyms T-SOD
Sample type Serum,plasma,urine,cells,cell culture supernatant,tissue
Sensitivity 2.4 U/mL
Detection range 2.4-61 U/mL
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 70 min
Precision Average inter-assay CV: 5.6%Average intra-assay CV: 5.5%
Other instruments required Micropipettor, Centrifuge, Incubator
Other reagents required PBS (0.01 M, pH 7.4), Normal saline (0.9% NaCl)
Storage Reagent 4: -20℃, others: 2-8℃
Valid period 6 months

Manual MSDS

Additional information

CatalogID

E-BC-K019-M-48, E-BC-K019-M-96