These are some of the top questions surrounding gel electrophoresis and agarose gels. Learn about how to reuse/remelt your gels, how to calculate concentration, what volume gel to use for your specific tray, and more.1.Can agarose be reused/remelted? Agarose can...

Reverse transcription is a technique used by researchers to generate a complementary strand of DNA (cDNA) from RNA. The technology is based on a retroviral mechanism whereby the enzyme reverse transcriptase can reverse transcribe RNA into DNA. This is especially...

T7 endonuclease I is a popular and very useful enzyme that is often used to quantify a targeted mutation. Here, we will describe the mechanism of this powerful nuclease and guidelines for its use in genome editing.Powerful genome editing technologies like CRISPR,...

Sometimes an eyebrow raise is enough to melt our hearts. That is exactly what happens every day to many dog owners as their furry friends look at them with those “puppy eyes.” This seemingly simple facial movement dogs make has taken thousands of years to evolve and...

Genetic research has exploded in recent decades with emerging technologies, breakthroughs in sequencing and the advancing sophistication of PCR. This brief overview examines a few applications or areas of genetic research and how PCR is used in these types of...

It’s just after sunset. The air is cooler. The sky is a mix of pink, purple and blue. Cicadas loudly stridulate. Between two widely spread boughs of a tall spruce tree, an orb-weaver spider spirals round and round, meticulously weaving her web. It’s hard to stop...

Glucose, a small six-carbon molecule, is elemental to life. Upon consuming it, cells metabolize it, releasing the energy needed for all biological processes. Normal physiological function, including functioning of neurons in the human brain, depends on effective...

You’ve got a cat or few. They love snuggles and cuddles. They’re super warm and soft and perfect. Life is good. Then something comes up, a significant other, a baby on the way, a roommate, and they have a horrible cat allergy. What do you do?About 3.2 million cats in...

Since the second that Darwin stepped off the S.S. Beagle exhilarated by thoughts of how species diversity occurs, he received criticism. Now that the scientific community has had some time (over 180 years to be exact) to wrap their heads around his theory, new ideas...

“Lois, everyone knows there are only two things that can survive a [nuclear] holocaust: cockroaches and Twinkies…” Not only is this idea perpetuated in season two’s “Da Boom” episode of “Family Guy,” but it’s also a subplot for Woody Harrelson’s character in 2009’s...

What is Mycoplasma?Cell culture is a cornerstone technique for biologicalresearch laboratories. Cell culture is essential for studying cellularregulatory mechanisms, for stem cell and regenerative medicine studies, and forthe generation of biologically active...

As the end of 2019 nears, we look back at the many impactful scientific breakthroughs of this past year. We hope this collection will inspire others to continue on their quest for knowledge and discovery. Cellular Function can be Restored in Pig Brain Hours After...

The southern journey of the monarch butterfly is probably one of the longest routes taken for any insect migration. Yet, it is crucial for their survival. Their behavior and reactions to environmental signals also tell researchers a lot about other things happening in...

What are competent cells?Cell competence refers to a cell’s ability to take up foreign (extracellular) DNA from its surrounding environment. The process of genetic uptake is referred to as transformation. In some cases, the genetic material taken in by a cell can...

In the long history of biology, there have been so many amazing discoveries! Advancements in the origins of life, the mechanisms of life, cures for new (and some rediscovered) maladies, and so many other interesting things in between are what consistently make biology...

Since the early 1970s, restriction enzymes have become an important part of cloning and many other applications, including DNA mapping. Restriction enzymes are enzymes that cut DNA at or near a specific target sequence, called a restriction site.Most of the time, as a...

olecular cloning allows us to study genes, modify genes, reintroduce modified genes into the natural host or into another host, or to overexpress a recombinant protein. You can perform molecular cloning by incorporating a piece of DNA into a vector, such as a plasmid...

Have you ever caught fireflies in the summer and watched as they crawl over your hands? It’s fascinating to watch them flashing their light in the dark.In the late 1940s, Dr. William McElroy, a scientist at Johns Hopkins University, was curious about this natural...

It’s Friday, 6:57 p.m. in the newsroom, and no one wants to be there. The atmosphere has shifted from being as casual as happy hour to being as tense as a DMV visit. The weekly meeting is now nearing its two-hour mark. Burnt out reporters and editors are either...

Protease inhibitors and protease inhibitor cocktails are a useful reagent during protein research applications. In this article, we'll take a look at what protease inhibitors and cocktails are, how to prepare your cocktails, when to use them, and so much more. Along...

Fatigue is among the earliest and common symptoms of an illness. In fact, most patients complain about fatigue at the time of hospital admission.Fatigue can be associated with various health problems, such as anemia, hyperthyroidism, electrolyte imbalance, diabetes,...

Often, enzymes such as proteinase K are evaluated heavily for their activity. Since the role of an enzyme is to hasten a chemical reaction, understanding enzymatic activity gives researchers a better idea of how well they will perform. The second component for...

Article ContentsWhat is TCEP?Sulfhydril Groups and Disulfide Bridges in ProteinsTCEP and Reduction ReactionsTCEP or DTT?Buffers to Dissolve TCEPHow to Prepare 0.5 M TCEP Stock SolutionRelated ProductRelated ArticlesReferencesWhat is TCEP?Tris (2-carboxyethyl)...