Description
GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!
Recombinant Human Vascular Endothelial Growth Factor165
VEGF165
VEGF165 is an abundant glycosylated cytokine composed of two identical 165 amino acid chains in the Vascular Endothelial Growth Factor family. It plays a significant role in stimulating angiogenesis, not only during embryonic development, but also upon injury to vascular tissue or when blockage occurs. This growth factor is the most abundant and potent form in its family and uses a heparin-binding mechanism to induce angiogenesis. It has been shown to play a role in tumor metastasis by vascularizing metastatic tissues and to increase capillary permeability. In addition, VEGF165 tightly binds receptor tyrosine kinases Flk-1 and Flt-1. Recent studies implicate VEGF165 in production of osteoclasts in murine bone marrow.
Formulation: Sterile filtered white lyophilized powder. Purified and tested for use in cell culture.
Sequence: MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQENPCGP CSERRKHLFV QDPQTCKCSC KNTDSRCKAR QLELNERTCR CDKPRR
MW: ~38.6 kDa (2×166 amino acid) (A disulfide linked homodimeric protein with two 166 amino acid polypeptide chains)
Purity: >95 % by SDS-PAGE and HPLC analyses.
Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a assay using umbilical vein endothelial cells (HUVEC) is between 1.0-8.0 ng/ml.
Accession Number: P15692
HGNC ID: 12680
Source: Escherichia coli
Storage/Handling: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage. The reconstituted sample can be apportioned into working aliquots and stored at -80°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Reconstitution: The sample should be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a siliconized tube using PBS that contains a 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Reconstituted solutions are stable for up to one week at 2-8°C. Stock solutions should be aliquoted and stored at -80°C. Further dilutions should be made in appropriate buffered solutions.
Additional resources for this product may be found at:
NOT FOR HUMAN USE!
Note: 1000 µg volume may be delivered in multiple containers.
