Description
| Description | Ac-DEVD-AFC is a fluorogenic substrate |
| In vitro | Caspase activation was measured using the fluorogenic compound N-acetyl-asp-glu-val-asp-7-amino-4-trifluoromethyl coumarin (Ac-DEVD-AFC). We show that this substrate rapidly enters cells where it is efficiently cleaved at the aspartate residue by specific caspases, yielding the fluorescent compound 7-amino-4-trifluoromethyl coumarin (AFC). Following cell disruption, released AFC was separated on HPLC and detected by fluorescence. The appearance of AFC in cells was blocked by the pancaspase inhibitor benzyloxycarbonyl-val-ala-asp-fluoromethylketone, thus establishing that intracellular caspases were responsible for the cleavage. Caspase activity was first noted after about 2 h of incubation with doxorubicin or dactinomycin, the production of AFC being linear with time afterward[1]. |
| Molecular Weight |
729.61 |
| Formula | C30H34F3N5O13 |
| CAS No. | 201608-14-2 |
Storage
keep away from moisture
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Solubility Information
DMSO: 40 mg/mL (54.82 mM)
H2O: insoluble
