Ampicillin (Sodium), USP Grade

Ampicillin sodium is a member of the extended-spectrum β-lactam family and similar in structure to penicillin. It is used in the selection of antibiotic cell hybrids and has a high rate of action against many gram-negative bacterial strains. Ampicillin sodium targets non-ESBL (Extended Spectrum β-lactamase) bacteria including
Staphylococcus and Streptococcus species and medically important enteric pathogens such as Shigella and Salmonella. It has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus species. Resistance to ampicillin is routinely utilized as a selectable marker to confirm successful cell transformation. Ampicillin Sodium is freely soluble in water.

β-lactams inhibit the formation of peptidoglycan cross-links within bacterial cell walls by targeting penicillin-binding proteins or PBPs. Consequently, the bacterial cell wall becomes weak and cytolysis occurs. Resistance to β-lactam antibiotics occurs in the presence of cells containing plasmid encoded extended spectrum β-lactamases or ESBLs.

Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.

Product Specifications
Sodium Ampicillin

Formula: NaC₁₆H₁₈N₃O₄S

MW: 371.39 g/mol

Storage/Handling: Store desiccated at -20°C. Soluble in water.

PubChem Chemical ID: 23663979

Ampicillin Stock Solution – 100 mg/ml

Instructions

1. Weigh 1 g of Ampicillin (Ampicillin (Sodium), GoldBio Catalog # A-301 [CAS 69-52-3, mw.= 371.4]).
2. Add 10 ml of sterile H₂O. Dissolve completely.
3. Prewet a 0.22 μm syringe filter by drawing through 5-10 ml of sterile H₂O and discard water.
4. Sterilize Ampicillin Stock through the prepared 0.22 μm syringe filter.
5. Stock may be kept at -80°C for 1 year.
   • Note: Ampicillin solutions degrade 13% after one week at -20°C.