FREE SHIPPING IN GERMANY ON ORDERS OVER € 250 & € 500 ACROSS EUROPE!

HEPES, Sodium Salt

From: 55.50

Clear
SKU: N/A Category:

Description

HEPES sodium salt is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.8 – 8.2. HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. It is commonly used in cell culture media and as an ampholytic separator to create a pH gradient in isoelectric focusing. HEPES interferes with reactions between DNA and restriction enzymes to a lesser extent than similar buffers with less substituted amine groups, such as Tris. This lack of interference occurs because steric hindrance of the protonated tertiary amine in HEPES shields the negative charge of DNA to a lesser extent than less substituted buffers. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. It is suitable for use in the bicinchoninic acid (BCA) assay.

HEPES sodium salt can be mixed with HEPES free acid to achieve the desired pH.

Product Specifications
HEPES, Sodium Salt
sodium;2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate

HIGH PURITY GRADE

Formula: C8H17N2O4SNa

MW: 260.29 g/mol

Storage/Handling: Store in a dry and well-ventilated place.

PubChem Chemical ID: 2724248

Catalog ID H-401
CAS # 75277-39-3
MW 260.29 g/mol
Grade HIGH PURITY GRADE
Storage/Handling Store desiccated at room temperature.
P Protocol
O Other
C Certificate of Analysis
S Safety Data Sheet

Additional information

CatalogID

H-401-1, H-401-100, H-401-2.5, H-401-5, H-401-50, H-401-500