Description
Detection principle
Lactate dehydrogenase (LDH) is an oxidoreductase. LDH catalyzes the conversion of lactate to pyruvic acid and back, as it converts NAD+ to NADH and back. LDH is composed of four subunits (tetramer). The two most common subunits are the LDH-M and LDH-H protein. LDH is released into the blood by cells after tissue damage or erythrocyte hemolysis. Extracellular LDH activity is used to detect cell damage or cell death.
Performance characteristics
| Synonyms | LDH |
| Sample type | Serum, plasma, tissue, cells |
| Sensitivity | 4 U/L |
| Detection range | 4-400 U/L |
| Detection method | Colorimetric method |
| Assay type | Enzyme Activity |
| Assay time | 70 min |
| Precision | Average inter-assay CV: 7.2%Average intra-assay CV: 2.2% |
| Other instruments required | Micropipettor, Vortex mixer, Water bath |
| Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage | 2-8℃ |
| Valid period | 6 months |
