Description
Detection principle
Free cholesterol produces 4-cholestenone and hydrogen peroxide under the oxidation of cholesterol oxidase. In the presence of 4-aminoamylpyridine and phenol, peroxidase catalyze hydrogen peroxide to form red quinone compounds of benzoquinone imine phenizone. The color depth of the generated quinones is directly proportional to the cholesterol content.
Performance characteristics
| Synonyms | FC |
| Sample type | Serum, plasma, tissue |
| Sensitivity | 0.07 mmol/L |
| Detection range | 0.07-24 mmol/L |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 20 min |
| Precision | Average inter-assay CV: 5.2%Average intra-assay CV: 1.9% |
| Other instruments required | Micropipettor, Incubator, Vortex mixer, Centrifuge |
| Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4), Isopropanol(AR) |
| Storage | 2-8℃ |
| Valid period | 6 months |
