Description
Detection principle
Myeloperoxidase reduces hydrogen peroxide to a complex. The complex react with o-dianisidine (as hydrogen donor) to produce a yellow product which has a maximum absorption peak at 460 nm. The activity of MPO can be calculated indirectly by measuring the OD value at 460nm.
Performance characteristics
Synonyms | MPO |
Sample type | Serum, plasma, animal tissue |
Sensitivity | 19.42 U/L |
Detection range | 19.42-893.31 U/L |
Detection method | Colorimetric method |
Assay type | Enzyme Activity |
Assay time | 65 min |
Precision | Average inter-assay CV: 7.3%Average intra-assay CV: 5.4% |
Other instruments required | Tubes, Micropipette, Vortex mixer, 37℃/60℃ Water bath |
Storage | 2-8℃ |
Valid period | 6 months |