Description
Acridine Orange (AO) is a cell-permeant dye that stains nucleic acids (RNA and DNA), providing a means of generically labeling nucleated cells in samples. When bound to DNA, AO can be excited with a 488nm laser and will fluorescently emit at a 525nm maximum, making it well-suited for detection with the PMT1 (525/45nm) channel of the Moxi GO II. The cells can be counterstained with Propidium Iodide (PI) to simultaneously determine the sample viability using the well-established membrane permeability metric.
Applications: Simultaneous live nucleated, dead cell counts
Cassette Types: Type MF-M, Type MF-S
Compatible Instruments: Moxi GO II
Intended Use Statement: For Research Use Only. Product is not for use in diagnostic procedures.
Number of Tests: 200
Protocol:
1. Mix 10 μl of cells (make sure there are less than 10 M cells/ml) with 90 μl of Moxi Cyte AOPI reagent
2. Incubate in the dark for 5 minutes
3. Select the GFP app on the Moxi Go II 488
4. Insert the cassette
5. Pipette 60 μl of sample onto the cassette
6. Close the door
Sample Type: mammalian cells, large yeast, large algae, protozoa, primary mammalian cells, immortalized adherent cells, non-ES cell types
Storage, Stability, and Handling: Store at 2-8 °C.
