Description
Recommended for use with Moxi Flow and Moxi GO II flow cytometers for rapid, accurate and precise cell viability, cell count, and cell volume measurements.
Applications: Cell Viability
Cassette Types: Type MF-M, Type MF-S
Compatible Instruments: Moxi Flow, Moxi GO II
Formulation: Phosphate-buffered saline, pH 7.4, containing 0.045% sodium azide, surfactant, propidium iodide
Intended Use Statement: For Research Use Only. Product is not for use in diagnostic procedures
Number of Tests: 110
Preservative: 0.045% sodium azide
Protocol
1a. For starting cell concentration of 0.1-5e6 cells/ml dilute 15 uL of sample with 135 uL of Orflo PI stock solution
1b. For starting cell concentration of 5-10M cells/ml dilute 7.5 uL of sample with 142.5 uL of Orflo PI stock solution
2. Incubate for 5 minutes in the dark at room temperature (25°C)
3. Select Viability Assay option on Moxi Flow touch screen
4. Insert cassette, close door and wait for auto-laser alignment to complete
5. Mix final solution by gently inverting tube 5-10x
6. Pipette 75µL of solution into cassette well
7. Close door of Moxi Flow unit
8. Test will run automatically in 8-10 seconds
Sample Type: Mammalian Cells, Large Yeast, Large Algae, Protozoa Primary Mammalian cells, immortalized adherent cells, non-ES cell types
Storage, Stability, and Handling: Store at 2-8°C.
