Description
Detection principle
Under the presence of G6PDH, glucose-6-phosphoric acid is oxidized to 6-PG. In this reaction, NADP+ is reduced to NADPH. Under the action of electron coupling reagent 1-MPMS, NADPH reducts wST-8 to form orange formazan, which has the maximum absorption peak at about 450 nm. Formazan generated in the reaction system is proportional to the activity of G6DPH in the sample.
Performance characteristics
| Synonyms | G-6-PD, G6PDH |
| Sample type | Serum, plasma, animal tissue |
| Sensitivity | 0.01 U/L |
| Detection range | 0.01-50 U/L |
| Detection method | Colorimetric method |
| Assay type | Enzyme Activity |
| Assay time | 20 min |
| Precision | Average inter-assay CV: 5.8%Average intra-assay CV: 2.1% |
| Other instruments required | Pipettor, Water bath, Centrifuge |
| Storage | -20℃ |
| Valid period | 6 months |
