Description
Detection principle
MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.
Performance characteristics
| Synonyms | MDA |
| Sample type | Serum,plasma,animal tissue |
| Sensitivity | 1.13 μmol/L |
| Detection range | 2.92-40 μmol/L |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 65 min |
| Precision | Average inter-assay CV: 7.2%Average intra-assay CV: 4.1% |
| Other instruments required | Micropipettor, Vortex mixer, Incubator, Centrifuge, Magnetic Stirrers |
| Other reagents required | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Glacial acetic acid (analytical reagent, acetic acid concentration ≥99.5%), Absolute ethanol |
| Storage | 2-8℃ |
| Valid period | 6 months |
