Description
Detection principle
Under the condition of weak acidity, non-esterified free fatty acids (NEFA) react with nantokite to form copper soap, which has a specific absorption peak at 715nm. The content of NEFA can be calculated indirectly by measuring the OD value at 715 nm.
Performance characteristics
| Synonyms | NEFA |
| Sample type | Animal tissue |
| Sensitivity | 0.15 mmol/L |
| Detection range | 0.15-1.5 mmol/L |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 30 min |
| Precision | Average inter-assay CV: 5.1%Average intra-assay CV: 3.3% |
| Other instruments required | Vortex mixer, Micropipettor, Multichannel transferpettor (300 μL) |
| Storage | 2-8℃ |
| Valid period | 6 months |
