Description
Detection principle
Sucrase catalyzes its substrate (sucrose) to produce glucose, which produces hydrogen peroxide under the action of glucose oxidase. Hydrogen peroxide reacts with chromogenic agent to produce red substance, which has a strong absorption peak at 505 nm. In a certain concentration range, It’s absorbance is proportional to glucose concentration. Therefore, the activity of sucrase can be calculated by measuring the OD value at 505 nm.
Performance characteristics
| Sample type | animal tissu |
| Sensitivity | 20 U/mL |
| Detection range | 20-2000 U/mL |
| Detection method | Colorimetric method |
| Assay type | Enzyme Activity |
| Assay time | 70 min |
| Precision | Average inter-assay CV: 6.5%Average intra-assay CV: 5.4% |
| Other instruments required | Micropipette, Vortex mixer, Centrifuge |
| Other reagents required | PBS (0.01 M, pH 7.4) |
| Storage | 2-8℃ |
| Valid period | 6 months |
