Description
Detection principle
Proline can react with acidic-ninhydrin to form stable red compound. The maximum absorption peak of the compound is at 520 nm. And the concentration of Pro can be calculated by measuring the OD value at 520 nm.
Performance characteristics
| Synonyms | Pro |
| Sample type | Plant tissue,honey |
| Sensitivity | 0.17 μg/mL |
| Detection range | 0.17-35 μg/mL |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 80 min |
| Precision | Average inter-assay CV: 5.4%Average intra-assay CV: 4.5% |
| Other instruments required | Water bath, Micropipettor, Vortex mixer, Balance, Centrifuge |
| Other reagents required | Acetic acid, Methylbenzene, Absolute ethyl alcohol |
| Storage | 2-8℃ |
| Valid period | 6 months |
