Description
Detection principle
Using coenzyme I as a hydrogen carrier, LDH catalyze lactic acid to produce pyruvate. Pyruvate reacted with 2, 4-dinitrophenylhydrazine to form pyruvate dinitrophenylhydrazone, which was red-brown in alkaline solution, and the color depth was proportional to pyruvate concentration. The activity of LDH could be calculated by measuring OD value.
Performance characteristics
| Synonyms | LDH |
| Sample type | Serum,plasma,hydrothorax,tissue,cells |
| Sensitivity | 6 U/L |
| Detection range | 6-1000 U/L |
| Detection method | Colorimetric method |
| Assay type | Enzyme Activity |
| Assay time | 45 min |
| Precision | Average inter-assay CV: 2.4%Average intra-assay CV: 1.8% |
| Other instruments required | Micropipettor, Multichannel pipette, Incubator |
| Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage | 2-8℃ |
| Valid period | 6 months |
