Description
Detection principle
The content of protein carbonyl increased after oxidation, and the carbonyl group reacted with 2, 4-dinitrophenylhydrazine to form a reddish brown precipitate. The absorbance can be measured at 370 nm after the precipitation is dissolved. The carbonyl content can be calculated indirectly.
Performance characteristics
| Sample type | Serum,plasma,hydrothorax,cell culture supernatant,tissue |
| Sensitivity | 0.02 nmol/mgprot |
| Detection range | 0.02-10 nmol/mgprot |
| Detection method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 240 min |
| Precision | Average inter-assay CV: 8.6%Average intra-assay CV: 4.5% |
| Other instruments required | Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge |
| Other reagents required | Absolute ethanol, Ethyl acetate |
| Storage | 2-8℃ |
| Valid period | 6 months |
